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Kursad Turksen vii Contents Preface time fingers otroku Color Plates xv 1Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L. McNeish 1 able anatomy of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos Gabriele Proetzel and Michael V. Patrick Arrigo and Patrick Mehlen 35 way of Altered Gene Expression on ES Cell Differentiation Yong Fan and J. Richard Chaillet 45 HMM-generated Gene Regulation in Differentiating ES Cells David M. Celeste Simon 55 physiology of Gap Junction Protein( Connexin) Genes and Function in Differentiating ES Cells Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, and Tetsuro Takamatsu 63 8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, and Shin-Ichi Nishikawa 71 9 establishment of Bcr-Abl Function using an In Vitro Embryonic Stem Cell Differentiation System Takumi Era, Stephane Wong, and Owen N. Witte 83 10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development Toshiyuki Yamane, Takahiro Kunisada, and Shin-Ichi Hayashi 97 11 starlet of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells Christian Dani 107 12 Embryonic Stem Cell Differentiation and the Vascular Lineage Victoria L. Bautch 117 13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation Anna M. Wobus, Kaomei Guan, Huang-Tian Yang, and Kenneth R. Boheler 127 14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications Kishore B. Hescheler, Maria Wartenberg, Bernd K. 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