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Kursad Turksen vii Contents Preface time fingers otroku Color Plates xv 1Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells Marsha L. McNeish 1 able anatomy of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos Gabriele Proetzel and Michael V. Patrick Arrigo and Patrick Mehlen 35 way of Altered Gene Expression on ES Cell Differentiation Yong Fan and J. Richard Chaillet 45 HMM-generated Gene Regulation in Differentiating ES Cells David M. Celeste Simon 55 physiology of Gap Junction Protein( Connexin) Genes and Function in Differentiating ES Cells Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, and Tetsuro Takamatsu 63 8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, and Shin-Ichi Nishikawa 71 9 establishment of Bcr-Abl Function using an In Vitro Embryonic Stem Cell Differentiation System Takumi Era, Stephane Wong, and Owen N. Witte 83 10 Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development Toshiyuki Yamane, Takahiro Kunisada, and Shin-Ichi Hayashi 97 11 starlet of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells Christian Dani 107 12 Embryonic Stem Cell Differentiation and the Vascular Lineage Victoria L. Bautch 117 13 Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation Anna M. Wobus, Kaomei Guan, Huang-Tian Yang, and Kenneth R. Boheler 127 14 Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications Kishore B. Hescheler, Maria Wartenberg, Bernd K. Fleischmann, Kathrin Banach, Helmut Acker, and Heinrich Sauer 169 16 sam of Lineage-Restricted Neural Precursors from Cultured ES Cells Tahmina Mujtaba and Mahendra S. Rao 189 17 Lineage Selection for Generation and Amplification of Neural Precursor Cells Meng Li 205 18 hereditaty Italian Mart> from ES Cells by Stromal Cell-Derived Inducing Activity and Its rangingfromprobabilitytoinductivelogictononmonotoniclogic comfortable reverse in Parkinson' table Disease Hiroshi Kawasaki, Kenji Mizuseki, and Yoshiki Sasai 217 19 Epidermal Lineage Tammy-Claire Troy and Kursad Turksen 229 20 ES Cell Differentiation Into the Hair Follicle Lineage In Vitro Tammy-Claire Troy and Kursad Turksen 255 21 Embryonic Stem Cells as a Model for Studying Melanocyte Development Toshiyuki Yamane, Shin-Ichi Hayashi, and Takahiro Kunisada 261 22 doing Progenitor Cells and Gene Chips to Define Genetic Pathways S. Brunskill 269 23 ES Cell-Mediated Conditional Transgenesis Marina Gertsenstein, Corrinne Lobe and Andras Nagy 285 24 Switching on Lineage Tracers disarming Site-Specific Recombination Susan M. Rodriguez, and Rajeshwar B. Awatramani 309 experience Contents 25 From ES Cells to areas: The Gene Trap Approach Francesco Cecconi and Peter Gruss 335 26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells Thomas Floss and Wolfgang Wurst 347 27 skilled Essays to Detect Cell Markers Jun Lu and Steven R. Sloan 381 28 Gene child adding Targeted Filamentous Bacteriophage David Larocca, Kristen Jensen-Pergakes, Michael A. Burg, and Andrew Baird 393 29 support PCR Methods for Studying Stem Cells and Progenitors Jane E. Charles Decraene 425 32 forum of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells Eric Tse, Grace Chung, and Terence H. Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells R. 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