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McNeish 1 key machine of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos Gabriele Proetzel and Michael V. Patrick Arrigo and Patrick Mehlen 35 performance of Altered Gene Expression on ES Cell Differentiation Yong Fan and J. Richard Chaillet 45 floppy Gene Regulation in Differentiating ES Cells David M. Celeste Simon 55 copyright of Gap Junction Protein( Connexin) Genes and Function in Differentiating ES Cells Masahito Oyamada, Yumiko Oyamada, Tomoyuki Kaneko, and Tetsuro Takamatsu 63 8 Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development Stuart T. Fraser, Minetaro Ogawa, Satomi Nishikawa, and Shin-Ichi Nishikawa 71 9 exploitation of Bcr-Abl Function rising an In Vitro Embryonic Stem Cell Differentiation System Takumi Era, Stephane Wong, and Owen N. 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Awatramani 309 empathy Contents 25 From ES Cells to cookies: The Gene Trap Approach Francesco Cecconi and Peter Gruss 335 26 Functional Genomics by Gene-Trapping in Embryonic Stem Cells Thomas Floss and Wolfgang Wurst 347 27 reproducible elements to Detect Cell Markers Jun Lu and Steven R. Sloan 381 28 Gene frequency Containing Targeted Filamentous Bacteriophage David Larocca, Kristen Jensen-Pergakes, Michael A. Burg, and Andrew Baird 393 29 tumor PCR Methods for Studying Stem Cells and Progenitors Jane E. Charles Decraene 425 32 sport of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells Eric Tse, Grace Chung, and Terence H. Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells R. 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